preadipocyte growth Search Results


preadipocyte growth medium supplement pack  (PromoCell)
95
PromoCell preadipocyte growth medium supplement pack
Preadipocyte Growth Medium Supplement Pack, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocyte growth medium supplement pack/product/PromoCell
Average 95 stars, based on 1 article reviews
preadipocyte growth medium supplement pack - by Bioz Stars, 2026-03
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preadipocytes  (PromoCell)
95
PromoCell preadipocytes
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Preadipocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocytes/product/PromoCell
Average 95 stars, based on 1 article reviews
preadipocytes - by Bioz Stars, 2026-03
95/100 stars
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gst π rabbit polyclonal antibody  (Boster Bio)
90
Boster Bio gst π rabbit polyclonal antibody
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Gst π Rabbit Polyclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst π rabbit polyclonal antibody/product/Boster Bio
Average 90 stars, based on 1 article reviews
gst π rabbit polyclonal antibody - by Bioz Stars, 2026-03
90/100 stars
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antibiotic  (PromoCell)
92
PromoCell antibiotic
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Antibiotic, supplied by PromoCell, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibiotic/product/PromoCell
Average 92 stars, based on 1 article reviews
antibiotic - by Bioz Stars, 2026-03
92/100 stars
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preadipocytes growth medium  (PromoCell)
93
PromoCell preadipocytes growth medium
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Preadipocytes Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocytes growth medium/product/PromoCell
Average 93 stars, based on 1 article reviews
preadipocytes growth medium - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
preadipocyte basal medium  (PromoCell)
93
PromoCell preadipocyte basal medium
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Preadipocyte Basal Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocyte basal medium/product/PromoCell
Average 93 stars, based on 1 article reviews
preadipocyte basal medium - by Bioz Stars, 2026-03
93/100 stars
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preadipocyte differentiation medium  (Lonza)
95
Lonza preadipocyte differentiation medium
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Preadipocyte Differentiation Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocyte differentiation medium/product/Lonza
Average 95 stars, based on 1 article reviews
preadipocyte differentiation medium - by Bioz Stars, 2026-03
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pgm  (PromoCell)
93
PromoCell pgm
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Pgm, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgm/product/PromoCell
Average 93 stars, based on 1 article reviews
pgm - by Bioz Stars, 2026-03
93/100 stars
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preadipocyte growth  (PromoCell)
93
PromoCell preadipocyte growth
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Preadipocyte Growth, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocyte growth/product/PromoCell
Average 93 stars, based on 1 article reviews
preadipocyte growth - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
pgm-2 preadipocyte growth medium-2 bullet kit pt-8002  (Lonza)
90
Lonza pgm-2 preadipocyte growth medium-2 bullet kit pt-8002
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Pgm 2 Preadipocyte Growth Medium 2 Bullet Kit Pt 8002, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgm-2 preadipocyte growth medium-2 bullet kit pt-8002/product/Lonza
Average 90 stars, based on 1 article reviews
pgm-2 preadipocyte growth medium-2 bullet kit pt-8002 - by Bioz Stars, 2026-03
90/100 stars
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preadipocyte growth medium  (Cambrex)
90
Cambrex preadipocyte growth medium
Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white <t>preadipocytes</t> were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.
Preadipocyte Growth Medium, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocyte growth medium/product/Cambrex
Average 90 stars, based on 1 article reviews
preadipocyte growth medium - by Bioz Stars, 2026-03
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Image Search Results


Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white preadipocytes were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.

Journal: Data in Brief

Article Title: Updating “Data on changes in lipid profiles during the differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatic tools” with data on the changes in protein profiles

doi: 10.1016/j.dib.2023.109036

Figure Lengend Snippet: Schematic of the experimental procedure. Schedule from cell culture to proteomic analysis. Human subcutaneous white preadipocytes were seeded in a 12-well plate on day 0. Twenty-four hours after seeding, spindle-shaped cells were observed. After approximately seven days, the preadipocytes achieved 100% confluence on preadipocyte growth medium (Stage-1). The preadipocytes were then differentiated for three consecutive days in preadipocyte differentiation medium (Stage-2). Three days after replacement with adipocyte nutrition medium, small lipid droplets appeared (Stage-3). The number of lipid droplets gradually increased (Stage-4) until the cytoplasm was filled with lipid droplets (Stage-5). Proteins were then extracted and cleaved with trypsin. The peptides were analyzed using liquid chromatography and mass spectrometry (LC-MS/MS). Data were analyzed using the Proteome Discoverer 2.2 using the Mascot 2.6 search engine.

Article Snippet: The preadipocytes were cultured in Promocell Preadipocyte Growth Medium until 100% confluence (stage-1).

Techniques: Cell Culture, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Partial Least Squares Discriminant Analysis (PLS-DA) of proteins among the five stages. Stage-1: subcutaneous preadipocytes, stage-2: after inducing adipocyte differentiation, stages-3 to -5: from initiation of lipid droplet formation to appearance of mature subcutaneous adipocytes (dependent on lipid droplet amount and formation). The ellipses represent the 95% confidence interval. This figure was generated using MetaboAnalyst 5.0.

Journal: Data in Brief

Article Title: Updating “Data on changes in lipid profiles during the differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatic tools” with data on the changes in protein profiles

doi: 10.1016/j.dib.2023.109036

Figure Lengend Snippet: Partial Least Squares Discriminant Analysis (PLS-DA) of proteins among the five stages. Stage-1: subcutaneous preadipocytes, stage-2: after inducing adipocyte differentiation, stages-3 to -5: from initiation of lipid droplet formation to appearance of mature subcutaneous adipocytes (dependent on lipid droplet amount and formation). The ellipses represent the 95% confidence interval. This figure was generated using MetaboAnalyst 5.0.

Article Snippet: The preadipocytes were cultured in Promocell Preadipocyte Growth Medium until 100% confluence (stage-1).

Techniques: Generated